| Group | Club/Team Name | Team ID | Head Coach |
| A1 | 402 Development Academy 402 DA 2009B Gold |
03136-0011B6U-0044 | Eli Brown |
| A2 | Gretna Elite Academy GEA ECNL-RL 09B |
02077-0011B6U-0133 | Abel Alvarado |
| A3 | Lex Soccer Unifut Lex United |
06130-0011B6U-2009 | Wilmar Galvan |
| A4 | Lincoln Surf Lincoln Surf 09B Premier |
03134-0011B6U-1823 | Giovanni Tapia |
| A6 | Omaha United Soccer Club OUSC 2009B BLACK |
03133-0011B6U-0103 | Elias Ramirez |
| A7 | Omaha Surf Omaha Surf 2009B Black |
02016-0011B6U-0041 | Ricardo Gomez |
| A8 | Sting Soccer Sting Nebraska B09 Elite Beckmann |
03137-0011B6U-0036 | James Beckmann |
| A9 | Omaha Surf Omaha Surf 2009B White |
02016-0011B6U-0040 | Devante Hartin |
| A10 | Sporting Nebraska FC Sporting Nebr B ECNL-RL 2009 |
03036-0011B6U-0043 | Hendry Rilantono |
| A11 | United Futbol Academy UFA DSM 2009B ELITE |
0422-01SB09-0033 | Carlos Smith |
| A12 | International Soccer Academy INTER - B16U |
05084-0011B6U-0008 | Nelson Ixcot |
| A13 | Elkhorn Athletic Association EAA NE ROOTS 09/10B I |
03004-0011B6U-0012 | Cesar Herrera |