| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Sporting Nebraska FC Sporting Nebr Storm II 09G |
03036-0013G6U-2335 | Julia Rodehorst |
| A2 | Bennington Soccer Club BENSC BLAZE 16G |
03057-0011G6U-0004 | Bob Marxsen |
| A3 | Sporting Nebraska FC Sporting Nebr G LNK Elite 2009 |
03036-0011G6U-0003 | Amanda Gangwish |
| A4 | Gretna Elite Academy GEA 09G Showcase |
02077-0011G6U-0087 | Christopher Mellott |
| A5 | Hawks FC Lady Hawks 2009 |
0N135-0011G6U-0007 | Dave Rus |
| A6 | Lincoln Spirit Soccer Club LSC Pulse 09G |
03024-0011G6U-3767 | Adam Bals |
| A7 | Elkhorn Athletic Association EAA NE ROOTS - 09/10G II |
03004-0011G6U-0023 | Prince Artey |
| A8 | Omaha United Soccer Club OUSC 2009G BLACK |
03133-0011G6U-0099 | Jose Munoz |
| A9 | 402 Development Academy 402 DA 2009G Gold |
03136-0011G6U-0046 | Haedar Kasem |