| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Sporting Nebraska FC Sporting Nebr G Acad Ajax 2016 |
03036-0011G9U-2293 | Matt Bragg |
| A2 | Omaha United Soccer Club OUSC 2016G BLACK |
03133-0011G9U-0092 | Enrique Almonte |
| A3 | Xtra Soccer Academy Xtra Soccer Academy Gold - G9U |
02079-0011G9U-0002 | Alanya Hannigan |
| A4 | 402 Development Academy 402 DA 2016G Premier |
03136-0011G9U-0013 | Alexander Meza |
| A5 | Gretna Elite Academy GEA Academy Pre-ECNL 16G II |
02077-0011G9U-0142 | Zack Brennan |